The PCR reactions/phylogenetic analysis project

LAB REPORT WRITING INSTRUCTIONS

Your lab report should include a written description of a selection of the experiments performed during the lab section of the class. Instead of writing weekly reports that contain everything that was done during a particular week, you should write a report that refers to our two majors experiment:
1. the PCR reactions/phylogenetic analysis project
2. the DNA cloning project.
These reports may be 2 to 4 page long each.

Both sections should include a title that reflect the nature of the experiment and follow a format that is consistent with the vast majority of scientific reports: introduction, methods, results and discussion. You may choose to separate these paragraphs by headings. The introduction may include a brief background of the techniques used and an explanation of potential (expected) results. The methods should be written as a paragraph and provide enough information for a student not enrolled in the class to understand what you did. The results may include a written description of what you obtained, as well as drawings/pictures. In the discussion, you should interpret the results of the experiments and compare them to what was expected. You should end each section by a general conclusion that highlights what you did and the results you obtained.

The write-up should be typed in Times New Roman Font, 12 pt, single-spaced. You do not have to include any references. Remember that both your handouts and textbook contain a lot of helpful information. PLEASE DO NOT PLAGIARIZE THESE SOURCES. If you choose to work with your lab partner(s), make sure that your reports do not end up identical/very similar, or you will be penalized. You will not be graded on the results you obtained or on the quality of your drawings. The large part of the grade will focus on the clarity of your writing: your lab report should reflect your personal understanding of the techniques we used and the protocols that we followed, as well as your abilities to summarize/describe in your own words the experiments you performed. You will also be graded based on the interpretation of the data you obtained.

The two major lab sections (PCR, Cloning) will be worth 50 points each, for a total of 100. For the phylogenetic analysis, you may access the sequence in a separate file available on Blackboard. You will need to identify your sequence, BLAST it, and see where it is on the phylogenetic tree. If you can not locate your own sequence, use a random student sequence or select a sequence labeled “MYSTERYSTUDENT”.

If you need additional instructions, please do not hesitate to contact either your TA or myself (aurelien@nova.edu).

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SECTION 1: PCR AMPLIFICATION AND PHYLOGENY OF mtDNA

INTRODUCTION

Blahblahblah blah blah

METHODS

Isolation of DNA: blah blah blah

PCR amplification: blah

Gel electrophoresis: blahblah

BLAST sequence analysis: blablabla

Phylogenetic analyses: blablabla

RESULTS

PCR amplification: The results of the amplification were seen as bands on the agarose gels. Figure 1 represents blah blablabla

BLAST analyses:

Phylogenetic analyses: The obtained phylogenetic tree is reported in Figure 2. It shows that blablabla. (Infer the phylogenetic tree from the mitochondrial control region sequences generated during the semester. Root the tree with the chimp sequence.)

DISCUSSION
Was your DNA successfully isolated and amplified? How can you tell?
Comment on the topology of the tree. Can you identify some major clades? Does the tree look like what you expected? What student (number) is most likely from African descent? What student is likely from Asian descent? European descent? Is Homo sapiens sapiens monophyletic in regards to Homo sapiens neanderthalensis ?

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The PCR experiments are fairly straightforward- the gene cloning experiment may be more complicated, as you need to explain where your PCR insert come from and how you produced it.

find the cost of your paper