Unknown lab report on proteus mirabilis

 

ab Report on Unknown
(10% of your grade)
Near the end of the semester you will be
given a culture of an unknown bacteria. It will be one of the bacteria that we have used
previously in the semester. You will use tests that we have used throughout the
semester to identify the bacteria.

A Guide to
Identifying Unknowns is given to you in the
Handout section of iCollege.
The second lab report will cover these tests and what your conclusions are for the
identification of the unknown.
Use the following format
:
Lab reports on Unknown should consist of
at least 5 pages. Failure to include any
of these pages will result in loss of points. IF any descriptions/directions from the
lab reports guidelines are included in a lab report points will be deducted.
Page 1
: Cover Sheet:
1
point
Name, Lab Meeting
Time & Days, Unknown Number & Organism
The Unknown Number
and Name
must be included on the cover sheet to get full
credit for your report.
Bacterial name must be written correctly: Genus name
capitalized first letter, species name lower case, all in italic
s or underlined.
Page 2
&3
: Gram Stain & Streak Plate:
2
points
Gram Stain: Should be drawn by hand in color.
Total Magnification
should also be
included.
Write out the method
you used
for Gram stain
(be
precise
when detailing the
times used, especially for decolorization)
in this section.
Describe your conclusion for
Gram reaction and cell morphology.
Streak Plate: Should be drawn by hand in color. The type of media & incubation
temperature should also be included.
Describe any conclusions for colony morphology
and color.
Page 4
: Materials and Methods:
2
pts
Include descriptions of all media used, inocu
lating techniques, incubation temperature
and time of incubation, reagents used for testing media or techniques used to read
results for each test presented in the Biochemical tests.
You do not have to describe
aseptic technique; it is enough to say that you used aseptic technique.
All
procedures
(except for Gram Stain)
go in this section
. Write in complete sentences
,
not lists
.
DO
NOT put results in this section.
6
Page
5
: Biochemical
Tests Chart
(RESULTS)
:
2
points
Biochemical test chart should begin with phenol red fermentation tests. Include all
media/tests performed
except
Gram stain
and streak plate
. The test chart should
include
phenol red fermentation tubes
and a minimum of 3
other tests
.
Do not include
Discussion in this section.
Page
5
: Biochemical Tests Chart: EXAMPLE
Test
Result
Interpretation
Phenol Red Broth
Glucose
Phenol Red Broth
Lactose
Phenol Red Broth
Sucrose
Yellow, no bubble
Pink, no bubble
Yellow, no
bubble
Organism was able to ferment glucose,
however, no gas was produced from
fermentation.
Organism was unable to ferment lactose
Organism was able to ferment sucrose, but
no gas is produced
Page
6
: Discussion
2
pts
The discussion should include the pro
cess you used to identity your unknown organism.
It should be written in third person, as a result the following terms should not be used;
I,
me, mine, or my
. Also, bacterial names must be written correctly. Bacterial genera
should
always
be capitalized and species should
always
be lower case. Both genus and
species should
always
be italicized when typed. Bacterial names may be abbreviated
after the complete name has been written one time in the discussion.
E. g.
Clostridium sporogenes
; a
fter abbreviation:
C. sporogenes
The discussion will likely begin with your Gram stain results.
Tell exactly how you
eliminated other organisms in determining the identity of your unknown.
You should
discuss any problems encountered throughout the proces
s, such as difficulty Gram
staining or results of any tests that did not turn out as expected. No procedures should
be included in the discussion.
Finally do a little research and report on your organism
and how it might be important to identify it in a cl
inical situation.
Page
7
: References:
1
points
At least one reference needs to be included written in CBE style. The Atlas can be used
as a reference as well as any scientific journal or textbook. Please do not use the
Laboratory Handouts since this is not a
published resource. If an online reference is
used it can NOT be Wikipedia. Todar’s Online Textbook of Bacteriology is acceptable. If
you have questions about the validity of a reference contact the instructor before using
the source

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