Introduction to Biochemistry

Posted on May 1, 2022 | by Dan

(1) Which of the double-stranded DNA sequences below will require more heat to
separate the two strands (select Option A or Option B)? (2) Why is more heat is required
to separate the strands?
Option A
5’-GGACTCCCGGGTGAG-3’
3’-CCTGAGGGCCCACTC-5’
Option B
5’-AACTGTTTAAAGACA-3’
3’-TTGACAAATTTCTGT-5’
(3) What will be the mRNA sequence that is produced during the transcription of the
DNA sequence below? Hint: As we discussed in class, there is a slight difference in the
nucleotides used in DNA vs RNA.
5’-ACGTCAGGTTTCCGT-3’
(4) Based on what was discussed in Lecture 9, name four (4) enzymes/proteins that play
a role in human DNA replication, and briefly describe the specific role each plays during
human DNA replication.

Sample Solution

tantly, is to ensure that the senior management are best equipped to lead the organisation successfully. However, further down the hierarchy Fielder’s contingency theory begins to hold much less relevance, it becomes impractical from a organisational perspective due to the number of people at this level of leadership. The logistics of matching the leader with their least preferred co-worker is impossible to consistently achieve, so a more continuum based approach is required. Figure 1: Chelladurai’s Multi-Dimensional Model of Leadership (Miller and Cronin, 2012) There are other contingency theories that provide a more continuum based approach such as Redding’s theory of leadership and management, however Fielder’s description of how situational factors affect the leadership style required for the situation is extremely useful in understanding the fundamentals of leadership (Pettinger, 2007). Chelladurai in his Multi Dimensional Model of Leadership, expands on much of Fiedler’s theory but in a continuum based approach, in which the leader can adapt their leadership style to fit the situation (Chelladurai and Madella, 2006). Chelladurai’s theory is taken from sports psychology but can be applied to an organisational scenario. It provides a much more empirical categorisation of task structure, clearly differentiating a plethora of situations that require certain leadership styles for success. Chealldurai found three characteristics that affect the leadership style required for a situation, called antecedents, they mainly expand upon Fiedler’s situational factors and leader – member relations and ultimately affect how a leader should behave towards a situation. The first are situational characteristics, the environment in which the leader must perform, the second are leader characteristics, the experience, personal qualities and skills of the leader, and the third are member characteristics, the motivation, skill and experience levels of group members (Chelladurai and Madella, 2006). The situational characteristics and member characteristics have a required behaviour to ensure maximum group performance, they also have a preferred behaviour to ensure the satisfaction of group members, if the leaders actual behaviour matches both the required behaviour and preferred behaviour o

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