Agarose Gel Electrophoresis
DNA extraction and Separation of DNA Fragments by Agarose Gel Electrophoresis
-Was the DNA isolated from DNA extraction “pure” DNA? Explain. (swishing water to
get cheek cells and mix it with detergent, sodium chloride and ethanol : DNA
extraction)
-What other cellular molecules might be in the DNA that you obtained? How could you
test for the presence of other types of molecules?
Why do certain molecules migrate towards the positive electrode, and other
molecules might migrate towards the negative electrode? How does the gel separate
DNA fragments based on their size? Where on the gel do you find larger fragments?
Where on the gel do you find smaller fragments?