started. Along these lines, information from each of the 30 trees couldn’t be gathered in that frame of mind because of wellbeing concerns. To forestall this meaningfully affecting the information, it was guaranteed information was gathered on successive days. Information was gathered utilizing an ISO-TECH ILM-01 Light Meter (RS Parts Ltd, Northants, Joined Realm) which estimates in Lux. By gathering north of about a month, the moon cycle had the option to be considered. To decide the request wherein light information would be gathered, an irregular number generator was utilized, permitting the disposal of examiner inclination. By utilizing an irregular number generator, trees were visited at various seasons of night, forestalling results being impacted by reoccurrances, for example, traffic signals. Utilizing the photograph taken when the handouts were gathered, the light meter was situated under the mark of the tree the flyers were gathered from. The light meter was then held at a manageable distance forthright at which the leaves were taken from. On the off chance that the assortment focuses were near the ground, the light meter was as yet held underneath the branches so it might in any case be held out at a careful distance. Holding the light meter out at a careful distance forestalled shadows being projected across the peruser and influencing the perusing. At the point when the trees were by a street, information was gathered when the street was obvious from vehicles to forestall any additional light being projected on the peruser and influencing the information. When the light information was all recorded, midpoints for each tree were determined. The fourth arrangement of information was limited because of no leaves being left on the tree, expanding how much light that would arrive at the assortment focuses. A super moon occasion likewise happened on the most recent two days of assortment, it was not normally present to add additional light that. 2.4 Picture Examination The imaging programming ImageJ (U. S. Public Organizations of Wellbeing, Bethesda, Maryland, USA) was utilized to break down the flyer photos that had been taken. A worldwide scale bar was set for all handouts utilizing the ruler from the photo. The length of the flyer was estimated from the base to the tip. The region of the flyer was additionally estimated by parting the variety channels and utilizing either red or blue, contingent upon what was best for that particular handout. Contrast levels were changed until the flyer was generally similar variety and afterward the particles were investigated, giving a precise region of the pamphlet. On the off chance that there was harm to the pamphlets, for example, tears or openings, the region of the excess flyer was estimated. Notwithstanding practically all flyers being contaminated with the parasite Guignardia aesculi, the subsequent leaf smear from this disease was overlooked. To gauge the contaminated regions, the freehand apparatus was utilized to draw around the mined region of the handout. The extent of leaf digger contamination per flyer was then determined. When each pamphlet had been broke down, the normal extent of leaf excavator disease per tree was determined. By involving the tree as the unit of replication, pseudoreplication was not a variable. 3. Results>