Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography-mass spectrometry (LC-MS(/MS)) instruments ( assignments)

Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography-mass spectrometry (LC-MS(/MS)) in" rel="nofollow">instruments ( assignments)- Please who will work in" rel="nofollow">in this paper must be have knowledge in" rel="nofollow">in GC-MS and LC-MS and Forensic toxicology. - language must be simple and clear without complicated words. - answer 11 questions with basics. - any answers please provide references use in" rel="nofollow">in pdf or web site. - Any questions will be welcomed before fin" rel="nofollow">inish the paper to check the answer that done. - check the word I will uploaded and I will post the questions here also. The questions are: 1- Briefly describe the ionisation mechanisms for ESI, APCI and APPI. - Describe with 150 words for each ( ESI, APCI and APPI). Total 450 words and separated in" rel="nofollow">in 3 paragraphs. 2- Briefly describe how the followin" rel="nofollow">ing mass analysers filter / separate ions based on their mass-to-charge ratio; ion trap, quadrupole and time-of-flight? - Describe it in" rel="nofollow">in 275 words for the three type please. 3- Explain" rel="nofollow">in what isocratic and gradient elution is, in" rel="nofollow">include when you may need to use either type of elution and comment on their benefits? - Explain" rel="nofollow">in in" rel="nofollow">in 220 words please. 4- Compare reversed phase versus normal chromatography and highlight the main" rel="nofollow">in differences between the two types of chromatographic separation? - Explain" rel="nofollow">in in" rel="nofollow">in 220 words please. 5- Draw a fully labelled schematic EI ion source diagram? - Draw 6- Briefly describe the ionisation mechanisms for both EI and CI ? - 120 words for each EI and CI ( total 240) 7- Explain" rel="nofollow">in the importance of air/water checks and tunes in" rel="nofollow">in GC/MS ? - explain" rel="nofollow">in in" rel="nofollow">in 150 words please. 8- For both positive and negative ionisation give at least two examples of analyte precursor ion adducts that can be formed e.g. [M+?]+ - 100 words. 9- In addition to the notes on your observations durin" rel="nofollow">ing the laboratory, discuss the factors which you have altered and explain" rel="nofollow">in the effect they have had on the chromatographic separation? - The factors that has been altered ( oven temperature, temperature program ramp and in" rel="nofollow">inlet mode (splitless/ split). 150 words please 10- Based on its mass spectrum, discuss the use of diazepam-d5 as an in" rel="nofollow">internal standard in" rel="nofollow">in the analysis of diazepam? - 50 words. 11- Explain" rel="nofollow">in why morphin" rel="nofollow">ine has to be derivatised prior to analysis by GC/MS. - 75 words.