Lab Report for the Protein Module
– Lab Report for the Protein" rel="nofollow">in Module
write up a ‘Results and Discussion’ section for your lab report. The lab report covers all of the experiments performed in" rel="nofollow">in the Protein" rel="nofollow">in Module (Weeks #5-#7). See the Lab Report Guidelin" rel="nofollow">ines on p. 5-8 of your lab manual for more in" rel="nofollow">information about the format and gradin" rel="nofollow">ing of the lab report. The lab report is due at the begin" rel="nofollow">innin" rel="nofollow">ing of lab next week.
In your lab report make sure to in" rel="nofollow">include the followin" rel="nofollow">ing:
Data to in" rel="nofollow">include:
1. BCA assay standard curve (from week 5)
2. A table with the concentration of your plasma sample
3. A picture of your protein" rel="nofollow">in gel (make sure the lanes are labeled)
4. A table with the measured sizes of the molecular weight markers
5. Molecular weight marker standard curve
6. Tables of protein" rel="nofollow">in sizes that were calculated for the plasma and antibody samples.
Discuss the followin" rel="nofollow">ing issues:
1. What was achieved by centrifugin" rel="nofollow">ing the blood sample that you received?
2. What was achieved by the affin" rel="nofollow">inity purification?
3. How many bands are present in" rel="nofollow">in the purified antibody sample?
4. What are the molecular weights of the bands in" rel="nofollow">in the purified antibody sample?
5. Why were there more bands in" rel="nofollow">in sample 1 than in" rel="nofollow">in sample 2?
6. What are the bands in" rel="nofollow">in sample 2?
7. Based on your experiment how many different protein" rel="nofollow">ins are there in" rel="nofollow">in an IgG antibody?
8. Did the Protein" rel="nofollow">in A capture all the antibody molecules in" rel="nofollow">in the origin" rel="nofollow">inal rabbit plasma sample?