Proximity of dyes

Why do you think proximity of dyes is required for a FRET signal? What happens when you increase the distance between the dyes? Why do you have to run an assay with the non-specific binding signal? What values are you getting when you subtract the non-specific signal from the total signal? What does finding the KD of this interaction tell you about the interaction? What kinds of compounds would you expect to have higher KD values? Lower KD values? Why would measuring the binding kinetics of a drug candidate be important in drug development? How can you use the competition graphs to determine how potent a drug compound is? Why does the graph from Experiment 8 look different from the graph in Experiment 7? What does finding the KI of this interaction tell you about the interaction? What kinds of compounds would you expect to have higher KI values? Lower KI values? In this experiment, we used the Dopamine Di receptor. What results would you expect to get with each compound (A, B, and C) if you were testing the Dopamine D2 receptor? What do these results means for cellular processes related to the Dopamine D2 receptor?      

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