Unknown lab report on proteus mirabilis

  ab Report on Unknown (10% of your grade) Near the end of the semester you will be given a culture of an unknown bacteria. It will be one of the bacteria that we have used previously in the semester. You will use tests that we have used throughout the semester to identify the bacteria. A Guide to Identifying Unknowns is given to you in the Handout section of iCollege. The second lab report will cover these tests and what your conclusions are for the identification of the unknown. Use the following format : Lab reports on Unknown should consist of at least 5 pages. Failure to include any of these pages will result in loss of points. IF any descriptions/directions from the lab reports guidelines are included in a lab report points will be deducted. Page 1 : Cover Sheet: 1 point Name, Lab Meeting Time & Days, Unknown Number & Organism The Unknown Number and Name must be included on the cover sheet to get full credit for your report. Bacterial name must be written correctly: Genus name capitalized first letter, species name lower case, all in italic s or underlined. Page 2 &3 : Gram Stain & Streak Plate: 2 points Gram Stain: Should be drawn by hand in color. Total Magnification should also be included. Write out the method you used for Gram stain (be precise when detailing the times used, especially for decolorization) in this section. Describe your conclusion for Gram reaction and cell morphology. Streak Plate: Should be drawn by hand in color. The type of media & incubation temperature should also be included. Describe any conclusions for colony morphology and color. Page 4 : Materials and Methods: 2 pts Include descriptions of all media used, inocu lating techniques, incubation temperature and time of incubation, reagents used for testing media or techniques used to read results for each test presented in the Biochemical tests. You do not have to describe aseptic technique; it is enough to say that you used aseptic technique. All procedures (except for Gram Stain) go in this section . Write in complete sentences , not lists . DO NOT put results in this section. 6 Page 5 : Biochemical Tests Chart (RESULTS) : 2 points Biochemical test chart should begin with phenol red fermentation tests. Include all media/tests performed except Gram stain and streak plate . The test chart should include phenol red fermentation tubes and a minimum of 3 other tests . Do not include Discussion in this section. Page 5 : Biochemical Tests Chart: EXAMPLE Test Result Interpretation Phenol Red Broth Glucose Phenol Red Broth Lactose Phenol Red Broth Sucrose Yellow, no bubble Pink, no bubble Yellow, no bubble Organism was able to ferment glucose, however, no gas was produced from fermentation. Organism was unable to ferment lactose Organism was able to ferment sucrose, but no gas is produced Page 6 : Discussion 2 pts The discussion should include the pro cess you used to identity your unknown organism. It should be written in third person, as a result the following terms should not be used; I, me, mine, or my . Also, bacterial names must be written correctly. Bacterial genera should always be capitalized and species should always be lower case. Both genus and species should always be italicized when typed. Bacterial names may be abbreviated after the complete name has been written one time in the discussion. E. g. Clostridium sporogenes ; a fter abbreviation: C. sporogenes The discussion will likely begin with your Gram stain results. Tell exactly how you eliminated other organisms in determining the identity of your unknown. You should discuss any problems encountered throughout the proces s, such as difficulty Gram staining or results of any tests that did not turn out as expected. No procedures should be included in the discussion. Finally do a little research and report on your organism and how it might be important to identify it in a cl inical situation. Page 7 : References: 1 points At least one reference needs to be included written in CBE style. The Atlas can be used as a reference as well as any scientific journal or textbook. Please do not use the Laboratory Handouts since this is not a published resource. If an online reference is used it can NOT be Wikipedia. Todar’s Online Textbook of Bacteriology is acceptable. If you have questions about the validity of a reference contact the instructor before using the source